Recombinant human cardiac troponin T (cTnT)

Cat.# 8RTT5

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  • Cat.#

    8RTT5

  • Synonym names

    Human cardiac troponin T adult isoform, human cardiac troponin T isoform #6 or TnT3

  • Source:

    Expressed in E. coli

  • Protein sequence:

    Product of gene TNNT2; 288 a.a.r.

    MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN ММHFGGYIQK QAQTERKSGK RQTEREKKKK ILAERRKVLA IDHLNEDQLR EKAKELWQSI YNLEAEKFDL QEKFKQQKYE INVLRNRIND NQKVSKTRGK AKVTGRWK

    Recombinant human cardiac TnT contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes).

  • Application:

    Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnT standard

  • Analysis:

    Purity > 95 % (SDS-PAGE)

    Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) - 0.490 (measured at 280 nm)

  • Immunologicalﾠ activity:ﾠ

    Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnT isoform

  • Purification:

    Immunoaffinity purification followed by ion-exchange chromatography

  • Presentation:

    Lyophilized from 0.01 M HCl

    It is recommended to reconstitute this product with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol), to a concentration close to 1 mg/ml.

  • Storage:

    Lyophilized -20°C (-15 … -30 °C allowed)
    Reconstituted -70 °C (-65 … -80 °C allowed)

  • Other information:

    Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

  • References:

    See all Troponin references

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Recombinant human cardiac troponin T (cTnT)

(Catalogue number and MAb: 8RTT5)

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Recombinant human cardiac troponin T (cTnT)

(Catalogue number and MAb: 8RTT5)

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