PAPP-A human, antibody

Cat.# 4P41

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  • Cat.#

    4P41

  • MAbs:

    10E1, 10E2, 5H9, 4G11, 3C8, 10H9, 11E4, 7A6, PAPP52

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human PAPP-A/proMBP (heterotetrameric PAPP-A, htPAPP-A) complex or purified human atherosclerotic tissue form of PAPP-A (MAb PAPP52).

  • Specificity:
    "

    Human PAPP-A antigen purified from placental blood (heterotetrameric complex, consisting of PAPP-A subunits and proMBP subunits). MAbs 11E4 and 5H9 are specific to proMBP subunit.

  • MAb isotypes:

    IgG1 for MAb PAPP52
    IgG2a for MAbs 4G11, 3C8, 10H9, 7A6
    IgG2b for MAbs 5H9, 11E4, 10E1, 10E2

  • Applications:

    htPAPP-A and dPAPP-A (PAPP-A dimer) immunoassays and immunoprecipitation.

    Recommended pairs for detection of total PAPP-A (htPAPP-A and/or dPAPP-A) (capture - detection):

    10E2 - 10E1
    4G11 - 3C8
    4G11 - 10H9
    10E1 - 7A6

    Recommended pairs for detection of human htPAPP-A antigen (capture  - detection):

    10E2 - 5H9
    5H9 - 10E2
    5H9 - 7A6
    10E1 - 11E4

    Please see Cat.# 4PD4 Data Sheet for recommended pairs for specific dPAPP-A antigen detection.

    PAPP-A immunodetection in Western blotting. MAbs 4G11 and 10E1 recognize htPAPP-A, consisting of PAPP-A subunits and proMBP subunits, in non-reducing conditions.

  • Purification:

    Chromatography on protein A Sepharose.

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN₃)

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

  • References:

    See other fertility products.
    Search PAPP-A references from PubMed.

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PAPP-A human, antibody

(Catalogue number and MAb: 4P41-)

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PAPP-A human, antibody

(Catalogue number and MAb: 4P41-)

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