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Specificity:
MAb E411 class specific for human IgE, Fc-region specific MAb 4F4 - IgE C?3-domain
specific MAb 5D4 - IgE C?2-domain specific MAb XTE4 - epsilon chain specific
.
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Storage:
+ 4 ᄚC
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Specificity:
Subclass-specific for human IgG1. No allotype restriction. Fc - region specific.
Specific antibody titer is 1/300 000 by indirect ELISA
.
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Presentation:
BS, pH 7.4, 0.1 % sodium azide (NaN3)
..
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Purification:
hromatography on protein G Sepharose
..
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Applications:
etection of IgG1 antibodies in ELISA. MAb 2C11 can be used for coating and as
conjugate. MAb 2C11 works in immunohistochemistry on cytostat sections.
..
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MAb isotypes:
gG1 for MAb 2C11
..
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MAbs:
C11 Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells
with spleen cells of Balb/c mice immunized with IgG from human serum.
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Other information:
Allergol. et Immunopathol. 1978, vol 1, pp. 423-426 Allergy and Clin. Immunol.
News, 1992, vol.4, 21-25 Cat. # 1G2
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Purification:
Abs 4F4, 5D4, E411, BE5, 4G7 and 4H10 by chromatography on protein A Sepharose
MAb XTE4 by ion exchange chromatography
..
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MAb isotypes:
gG for MAbs BE5, 4G7 and 4H10 IgG1 for MAb XTE4 and IEF6 IgG2a for MAbs 4F4,
5D4 and E411
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Cat.#
E4
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Storage:
+ 4 ᄚC
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Presentation:
Abs 4F4, 5D4, E411, BE5, 4G7, 4H10 and IEF6 in PBS, pH 7.4, 0.1 % sodium azide
(NaN3) MAb IEF6 by chromatography on protein G Sepharose XTE4 in 0.15 M NaCl, 10
mM Tris, pH 7.5, 0.05 % sodium azide (NaN3)
..