Anti-human adiponectin

Cat.# 2AN6

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  • Cat.#

    2AN6

  • MAbs:

    Adn23, Adn27, Adn36, Adn63, Adn94, Adn214, Adn222, Adn279, Adn305

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human recombinant adiponectin from E. coli (MAb Adn23) or with native human adiponectin (MAbs Adn27, Adn36, Adn63, Adn94, Adn214, Adn222, Adn279 and Adn305).

  • Specificity:
    "

    Human serum adiponectin

  • MAb isotypes:

    IgG1 for MAbs Adn63, Adn94, Adn214, Adn222, Adn279, Adn305
    IgG2a for MAbs, Adn23, Adn27, Adn36

  • Applications:

    MAbs Adn23, Adn63, Adn214 and Adn222 react with human serum adiponectin in Western blotting.

    MAbs Adn23 and Adn63 recognise the whole spectrum of oligomeric forms of adiponectin after non-heating, non-reducing SDS-PAGE.

    Recommended pairs for total adiponectin immunodetection in sandwich immunoassay (capture-detection):
      Adn36 – Adn27
      Adn63 – Adn94
      Adn94 – Adn63
    Adn305 – Adn279

    Recommended pairs for low molecular weight adiponectin immunodetection in sandwich immunoassay (capture – detection):
      Adn214 – Adn27
      Adn222 – Adn36

  • Purification:

    Chromatography on protein A Sepharose

  • Presentation:

    PBS, pH 7.4, 0.09 % sodium azide (NaN3)

  • Storage:

    +4 °C (+2 … +8 °C allowed)

  • References:

    Search references from PubMed.
    See other Adiponectin references.

    .
  • Material safety note:

    This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

    Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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