Tumor Marker Antibodies

Insulin-like growth factor binding protein 4 (IGFBP-4) antibody

Cat.# 4IGF4

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Cat.#

4IGF4
MAbs:

IBP144, IBP182, IBP190, IBP3, IBP154, IBP163, IBP180, IBP185
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant human IGFBP-4 expressed in mammalian cell line (MAbs IBP144, IBP182, IBP190, IBP180, IBP185, IBP154), synthetic peptide from human IGFBP-4 His142-Met156 (MAb IBP3) or synthetic peptide from human IGFBP-4 Lys157-Gln171 (MAb IBP163)
Specificity:

"
MAbs IBP144, IBP154 and IBP180:
human IGFBP-4; human N-terminal IGFBP-4 fragment (NT-IGFBP-4)
MAbs IBP182, IBP185 and IBP190:
human IGFBP-4; human C-terminal IGFBP-4 fragment (CT-IGFBP-4)
MAb IBP3:
human NT-IGFBP-4, cross-reaction with full-length human IGFBP-4 <5% (ELISA)
MAb IBP163:
human CT-IGFBP-4, cross-reaction with full-length human IGFBP-4 <5% (ELISA)
MAb isotypes:

IgG1 for MAbs IBP163, IBP190
IgG2a for MAbs IBP144, IBP154, IBP180
IgG2b for MAb IBP182, IBP185
IgG3 for MAb IBP3
Applications:

Detection of human IGFBP-4, NT-IGFBP-4, and CT-IGFBP-4. MAbs are working in direct ELISA and sandwich immunoassay.
Recommended pairs for human IGFBP-4 sandwich immunoassay (capture-detection):
IBP185 – IBP154
IBP182 – IBP144
IBP144 – IBP190
Recommended pairs for specific human NT-IGFBP-4 sandwich immunoassay (capture-detection):
IBP3 – IBP144
IBP3 – IBP180
Recommended pairs for specific human CT-IGFBP-4 sandwich immunoassay (capture-detection):
IBP182 – IBP163
Purification:

Chromatography on protein A Sepharose
Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage:

+4 °C (+2 … +8 °C allowed)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
References:

See all IGFBP-4 references.
Search other references from PubMed.
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Insulin-like growth factor binding protein 4 (IGFBP-4) antibody

Cat.# 4IGF4

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